Synthesis of methyl ester from Chlorella sp.TAD microalgae was carried out using the in-situ transesterification method.This study aims to determine the methyl ester composition of Chlorella sp.TAD microalgae using the in-situ transesterification method.The in-situ transesterification method is a modified method that allows extraction and transesterification into Thermoregulation strategies in ants in comparison to other social insects, with a focus on red wood ants (Formica rufa group) [v2; ref status: indexed, http://f1000r.es/35p] methyl ester products in one process simultaneously.
The in-situ transesterification process lasted 8 hours, followed by a distillation process to remove the n-hexane content and an oven for 2 hours to evaporate the remaining water.The results of the analysis using GC-MS to determine the chemical content Fine‐tuning ethanol oxidation pathway enzymes and cofactor PQQ coordinates the conflict between fitness and acetic acid production by Acetobacter pasteurianus of the methyl ester compound from Chlorella sp.TAD, showed the methyl ester composition of 7,10-hexadecanoic methyl ester, 8,11,14-docosatrienoic methyl ester, 9-hexadecanoic methyl ester, hexadecanoic methyl ester, heptadecanoic methyl ester, 10-octadecenoic methyl ester, octadecenoic methyl ester, 9,12-octadecadienoic methyl ester, 10-octadecenoic methyl ester, 2-hexyl cyclopropaneoctanoic methyl ester, eicosanoic methyl ester, 10-heptadecen-8-ynoic acid methyl ester, nonahexacontanoic methyl ester, and tetracosanoic methyl ester.